International Journal of Medical Laboratory Research (IJMLR)

DEVELOPMENT OF NEW SOLVENT SYSTEMS FOR THE ANALYSIS OF DIAZEPAM FROM BLOOD

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K Jaiswal, Vinod Kumar, Shiva Prasad

 

ABSTRACT: Diazepam is a medication of the benzodiazepine family that typically produces a calming effect. It is commonly used to treat a range of conditions including anxiety, alcohol withdrawal syndrome, muscle spasms, seizures, trouble sleeping, and restless legs syndrome. Diazepam was analyzed by using different instrumentation techniques such as HPLC,GLC,LC-MS,GC-MS etc. An attempt has been made to develop a new method for analysis of Diazepam in Biological sample such as blood using High Performance Thin Layer Chromatography (HPTLC) Plate. Diazepam was extracted from blood using liquid-liquid extraction method and analyzed by using High Performance Thin Layer Chromatography Plate. For Chromatographic separation, various binary and tertiary solvent systems were used as mobile phase. Fifteen different solvent systems with different volumetric ratios were used in separation and identification of Diazepam. Developed plates were viewed under UV light followed by spray of chromogenic reagents which successfully increased the sensitivity without dispensing the simplicity of the method. The method developed is a simple, rapid, expensive, non–destructive and reproducible which can be performed in any laboratory easily.

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KEY WORDS: Diazepam, Benzodiazepine, Solvent Systems, TLC, Rf, Spraying Reagent etc.

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