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DETERMINATION OF LC50 OF PHENOLIC COMPOUNDS (PHENOL & M-CRESOL) FOR A FISH, LABEO ROHITA

 

Vikash Gaur, Abhishek Mathur

 

ABSTRACT: Water pollution has become a global problem as various pollutants like heavy metals and toxic chemicals are discharged without prior treatment into the water. With the development of industrial production, a large volume of wastewater containing phenols was discharged into the aquatic environment. Moreover, chemical leakage further increased the emission of phenols into aquatic systems. Phenol and its methylated derivative (cresols) were selected due to their extensive use in industry and ecotoxicity to freshwater and marine organisms. This study focused on the ecotoxicity of phenol and m-cresol on aquatic systems. This paper emphasizes on the determination of 96hr LC50 value of phenol & m-cresol for the fish, Labeo rohita. The acute toxicity test was performed according to the standard methods in APHA and the value was calculated by probit analysis. The fish specimens were acclimatized in the laboratory conditions for 15 days. The stock solution of phenol & m-cresol was prepared and the fish fingerlings were treated with various concentrations ranging from 1 mg/l to 50 mg/l for 96 hours. The results showed that the median lethal concentration (LC50) of phenol & m-cresol for the fish, Labeo rohita is 3212 & 2957 mg/l. The susceptibility of Labeo rohita to the lethal effect of phenol & m-cresol were dependent on duration as well as on concentration. The mortality of the fishes is directly proportional to the concentration.

 

KEY WORDS: Phenol, Cresols Ecotoxicity, Biodegradation, Acute Toxicity, Labeo rohita, 96hr LC50.

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REFERENCES:

  1. J, Duda W. Phenols--Sources and Toxicity. Polish Journal of Environmental Studies. 2007 May 1;16(3).

  2. Saha NC, Bhunia F, Kaviraj A. Toxicity of phenol to fish and aquatic ecosystems. Bulletin of Environmental Contamination and Toxicology. 1999 Aug 1;63(2):195-202.

  3. Lopes PA, Pinheiro T, Santos MC, da Luz Mathias M, Collares-Pereira MJ, Viegas-Crespo AM. Response of antioxidant enzymes in freshwater fish populations (Leuciscus alburnoides complex) to inorganic pollutants exposure. Science of the total environment. 2001 Dec 3;280(1-3):153-63.

  4. Olaifa FE, Olaifa AK, Adelaja AA, Owolabi AG. Heavy metal contamination of Clarias gariepinus from a lake and fish farm in Ibadan, Nigeria. African Journal of Biomedical Research. 2004;7(3).

  5. Heath AG. Water pollution and fish physiology. CRC press; 1995 Sep 14.

  6. Dallas HF, Day JA. The effect of water quality variables on riverine ecosystems: a review. Freshwater Research Unit, University of Cape Town; 1993.

  7. Woodward DF, Brumbaugh WG, Delonay AJ, Little EE, Smith CE. Effects on rainbow trout fry of a metals-contaminated diet of benthic invertebrates from the Clark Fork River, Montana. Transactions of the American Fisheries Society. 1994 Jan 1;123(1):51-62.

  8. McKim JM. Evaluation of tests with early life stages of fish for predicting long-term toxicity. Journal of the Fisheries Board of Canada. 1977 Aug 1;34(8):1148-54.

  9. American Public Health Association, American Water Works Association. Standard methods for the examination of water and wastewater. American public health association; 1989.

  10. Finney DJ. Probit analysis. ed.

  11. Abdullah SA, Javed MU, Javid AR. Studies on acute toxicity of metals to the fish (Labeo rohita). Int. J. Agr. Biol. 2007;9:333-7.

  12. Javid AR, Javed MU, Abdullah SA. Nickel bio-accumulation in the bodies of Catla catla, Labeo rohita and Cirrhina mrigala during 96-hr LC50 exposures. Int. J. Agric. Biol. 2007;9:139-42.

  13. Adhikari S. Effect of calcium and magnesium hardness on acute copper toxicity to Indian

  14. major carp, Labeo rohita (Hamilton) and catfish, Channa punctatus (Bloch). Aquaculture Research. 2003 Oct;34(12):975-80.

  15. NEKOUBIN H, GHAREDAASHI E, IMANPOUR MR, MONTAJAMI S. Determination of LC50 of Lead Nitrate and Copper Sulfate in Caspian Roach (Rutilus rutilus caspicus). Walailak Journal of Science and Technology. 2012;9(4):333-40.

  16. Shah SL, ALTINDAĞ A. Effects of Heavy Metal Accumulation on the 96-h LC_50 Values in Tench Tinca tinca L., 1758. Turkish Journal of Veterinary and Animal Sciences. 2005 Mar 18;29(1):139-44.

  17. Witeska M, Jezierska B. The effects of environmental factors on metal toxicity to fish(review). Fresenius Environmental Bulletin. 2003;12(8):824-9

  18. Gaur V, Raina V, Jain R, Mathur A. 2016. Proceedings of International Conference on Recent Advances in Biotechnology & Nano biotechnology (Int- BIONANO-2016), pp. 101-115.

  19. Singh A, Jain DK, Kumar P. Determination of LC50 of cadmium chloride in Heteropneustes fossilis. GERF Bull Biosci. 2010;1(1):21-4.

  20. Gaur V, Mathur A. Effect of Phenolic

  21. compounds on atp’ases and metabolic profile of labeo rohita. RAPCS Proceeding 2017 pp: 49-60

  22. Gaur V, Mathur A. Evaluation of antioxidant profile of Labeo rohita in stress condition after exposure to phenolic compounds. Int. J. Sci. Res. Pub. 2017;7(6):423-34.

  23. Saarikoski J, Viluksela MA. Relation between physicochemical properties of phenols and their toxicity and accumulation in fish. Ecotoxicology and environmental safety. 1982 Dec 1;6(6):501-12.

  24. Ghosh TK. Effect of phenol pollution on aquatic life. Environment and Ecology. 1983;1(1):1-3..

  25. Alex A, Verma OP, Mathur A, Sharan A, Varshney PK. Evaluation of changes in metabolic parameters and enzymes involved in metabolic pathways in Clarias batrachus after exposure to phenolic compounds. Asian Journal of Biomedical and Pharmaceutical Sciences. 2013 Jun 12;3(21).

  26. Gad NS, Saad AS. Effect of environmental pollution by phenol on some physiological parameters of Oreochromisniloticus. Global Veterinaria. 2008;2(6):312-9.

  27. Arutchelvan V, Kanakasabai V, Elangovan R, Nagarajan S, Muralikrishnan V. Kinetics of high strength phenoldegradation using Bacillus brevis. Journal of Hazardous Materials. 2006 Feb 28;129(1-3):216-22.

  28. ATSDR, 2008 a. Toxicological Profile for Phenol. Agency for Toxic Substances and Disease Registry.

  29. ATSDR, 2008 b.Toxicological Profile for Cresols. Agency for Toxic Substances and Disease Registry.

  30. Avilez IM, Hori TS, de Almeida LC, Hackbarth A, Neto JD, da Cunha Bastos VL, Moraes G. Effects of phenol in antioxidant metabolism in matrinxã, Brycon amazonicus (Teleostei; Characidae). Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2008 Aug 1;148(2):136-42.

  31. Ayeni O. A preliminary assessment of phenol contamination of Isebo River in south-western Nigeria. Greener J. Phys. Sci. 2014;4(2):30-7..

  32. Basha KM, Rajendran A, Thangavelu V. Recent advances in the biodegradation of phenol: a review. Asian J Exp Biol Sci. 2010;1(2):219-34.

  33. Benville Jr PE, Korn S. THE ACUTE TOXICITY OF SIX MONOCYCLIC AROMATIC CRUDE OIL COMPONENTS TO STRIPED BASS {MORONESAXATIUS) AND BAY SHRIMP (CR A GO FRA N CISCO RU M) л.

  34. Cai W, Li J, Zhang Z. The characteristics and mechanisms of phenol biodegradation by Fusarium sp. Journal of Hazardous Materials. 2007 Sep 5;148(1-2):38-42.

  35. Cairns J, Buikema AL, Heath AG, Parker BC. Effects of temperature on aquatic organism sensitivity to selected chemicals.

  36. Call DJ, Brooke LT, Lu PY. Uptake, elimination, and metabolism of three phenols by fathead minnows. Archives of environmental contamination and toxicology. 1980 Nov 1;9(6):699-714.

  37. Chen, B., Guo, H., Su, R., Su, J., 1999. Analysing the quality of sea water from Meizhou Bay. J. Huaqiao Univ. (Nat. Sci.) 20 (4), 403–406 (in Chinese).

  38. Chen HR, Zhu LZ, Yang K, Ge F, Chen YY. Concentration and pollution characteristics of phenolic compounds in Qiantang River. China Environmental Science. 2005;25(6):729-32.

  39. Clemens HP, Sneed KE. Lethal doses of several commercial chemicals for fingerling channel catfish.

  40. Colgan PW, Cross JA, Johansen PH. Guppy behavior during exposure to a sub-lethal concentration of phenol. Bulletin of environmental contamination and toxicology. 1982 Jan 1;28(1):20-7.

  41. Dalela RC, Rani S, Rani S, Verma SR. Influence of pH on the toxicity of phenol and its 2 derivatives pentachlorophenol and dinitrophenol to some fresh-water teleosts. Acta Hydrochimica et Hydrobiologica. 1980 Jan 1;8(6):623-9.

  42. DeGraeve GM, Geiger DL, Meyer JS, Bergman HL. Acute and embryo-larval toxicity of phenolic compounds to aquatic biota. Archives of environmental contamination and toxicology. 1980 Sep 1;9(5):557-68.

  43. Duan W, Meng F, Lin Y, Wang G. Toxicological effects of phenol on four marine microalgae. Environmental toxicology and pharmacology. 2017 Jun 1;52:170-6.

  44. Ertürk MD, Sacan MT, Novic M, Minovski N. Quantitative structure–activity relationships (QSARs) using the novel marine algal toxicity data of phenols. Journal of Molecular Graphics and Modelling. 2012 Sep 1; 38:90-100.

  45. Fernando R, Salibián A, Ferrari L. Assessment of the pollution impact on biomarkers of effect of a freshwater fish. Chemosphere. 2007 Jul 1;68(8):1582-90..

  46. Gupta AB, Srivastava AK. Phenol induced changes in the carbohydrate metabolism of the Indian catfish Heteropneustes fossilis. Environmental biology of fishes. 1984 Apr 1;10(3):221-4.

  47. Gupta PK, Mujumdar VS, Rao PS, Durve VS. Toxicity of phenol, pentachlorophenol and sodium pentachlorophenolate to a freshwater teleost Lebistes reticulatus (Peters). Acta hydrochimica et hydrobiologica. 1982;10(2):177-81.

  48. Gupta, S., Dalela, R.C., Saxena, P.K., 1983b. Influence of temperature on the toxicity of phenol and its chloro‐and nitro‐derivatives to the fish Notopterus notopterus (Pallas). CLEAN–Soil Air Water 11 (2), 187–192.

  49. Hickey CW, Blaise C, Costan G. Microtesting appraisal of ATP and cell recovery toxicity end points after acute exposure of Selenastrum capricornutum to selected chemicals. Environmental Toxicology and Water Quality. 1991 Nov;6(4):383-403.

  50. Hodson PV, Dixon DG, Kaiser KL. Measurement of median lethal dose as a rapid indication of contaminant toxicity to fish. Environmental Toxicology and Chemistry: An International Journal. 1984 Apr;3(2):243-54.

  51. Kopperman HL, Carlson RM, Caple R. Aqueous chlorination and ozonation studies I. Structure-toxicity correlations of phenolic compounds to Daphnia magna. Chemico-biological interactions. 1974 Oct 1;9(4):245-51.

  52. Kotresha D, Vidyasagar GM. Isolation and characterisation of phenol-degrading Pseudomonas aeruginosa MTCC 4996. World Journal of Microbiology and Biotechnology. 2008 Apr 1;24(4):541-7.

  53. Lovell CR, Eriksen NT, Lewitus AJ, Chen YP. Resistance of the marine diatom Thalassiosira sp. to toxicity of phenolic compounds. Marine Ecology Progress Series. 2002 Mar 20; 229:11-18.

  54. Martínez-Gómez C, Vethaak AD, Hylland K, Burgeot T, Köhler A, Lyons BP, Thain J, Gubbins MJ, Davies IM. A guide to toxicity assessment and monitoring effects at lower levels of biological organization following marine oil spills in European waters. ICES Journal of Marine Science. 2010 Mar 25;67(6):1105-18.

  55. McLeay DJ. A rapid method for measuring the acute toxicity of pulpmill effluents and other toxicants to salmonid fish at ambient room temperature. Journal of the Fisheries Board of Canada. 1976 Jun 1;33(6):1303-11.

  56. Oruc EO, Sevgiler Y, Uner N. Tissue-specific oxidative stress responses in fish exposed to 2, 4-D and azinphosmethyl. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2004 Jan 1; 137(1):43-51.

  57. Pickering QH, Henderson C. Acute toxicity of some important petrochemicals to fish. Journal (Water Pollution Control Federation). 1966 Sep 1:1419-29.

  58. Phipps GL, Holcombe GW, Fiandt JT. Acute toxicity of phenol and substituted phenols to the fathead minnow. Bulletin of environmental contamination and toxicology. 1981 Jan 1;26(1):585-93

  59. Ruesink RG, Smith JR LL. The relationship of the 96-hour LC50 to the lethal threshold concentration of hexavalent chromium, phenol, and sodium pentachlorophenate for fathead minnows (Pimephales promelas Rafinesque). Transactions of the American Fisheries Society. 1975 Jul 1; 104(3):567-70.

  60. Stephenson RR. Effects of water hardness, water temperature, and size of the test organism on the susceptibility of the freshwater shrimp, Gammarus pulex (L.), to toxicants. Bulletin of environmental contamination and toxicology. 1983 Oct 1;31(4):459-66.

  61. Stoilova I, Krastanov A, Stanchev V, Daniel D, Gerginova M, Alexieva Z. Biodegradation of high amounts of phenol, catechol, 2, 4-dichlorophenol and 2, 6-dimethoxyphenol by Aspergillus awamori cells. Enzyme and Microbial Technology. 2006 Sep 4;39(5):1036-41.

  62. Tallur PN, Megadi VB, Ninnekar HZ. Biodegradation of p-cresol by immobilized cells of Bacillus sp. strain PHN 1. Biodegradation. 2009 Feb 1;20(1):79-83.

  63. Tatem HE, Cox BA, Anderson JW. The toxicity of oils and petroleum hydrocarbons to estuarine crustaceans. Estuarine and Coastal Marine Science. 1978 Apr 1;6(4):365-73.

  64. Verma SR, Rani S, Dalela RC. Synergism, antagonism, and additivity of phenol, pentachlorophenol, and dinitrophenol to a fish (Notopterus notopterus). Archives of environmental contamination and toxicology. 1981 May 1;10(3):365-70.

  65. Verma SR, Rani S, Tyagi AK, Dalela RC. Evaluation of acute toxicity of phenol and its chloro-and nitro-derivatives to certain teleosts. Water, Air, and Soil Pollution. 1980 Jan 1;14(1):95-102.

  66. Zhong W, Wang D, Xu X, Luo Q, Wang B, Shan X, Wang Z. Screening level ecological risk assessment for phenols in surface water of the Taihu Lake. Chemosphere. 2010 Aug 1;80(9):998

 To cite this article:

Gaur V, Mathur A; Determination of lc50 of phenolic compounds (phenol & m-cresol) for a fish, labeo rohita . Int. J. Med. Lab. Res. 2019, 4(1):55-63

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